Clinical significance of tumor mutation burden in patients after R 0 resection of colorectal cancer and capecitabine-based adjuvant chemotherapy / 中华普通外科杂志

Objective:To investigate the clinical significance of TMB among CRC patients after R 0 resection and capecitabine-based adjuvant chemotherapy. Methods:Data of 82 CRC patients were reviewed retrospectively. Tumor tissue specimens were collected for DNA extraction . Somatic mutation detection and TMB analysis were performed using next-generation sequencing (NGS) of tumor-related genes. The univariate analysis between TMB status and prognosis was carried out by Kaplan-Meier survival analysis and adjusted by multivariate COX regression analysis subsequently.Results:In these 82 cases,with the median follow-up period was 5.5 years the median disease-free survival (DFS) was 4.5 years, and the median overall survival (OS) was 5.7 years. The most common mutated somatic genes were TP53, APC, KRAS and PIK3CA, with the mutation frequencies of 68.3%, 64.6%, 46.3% and 29.3%, respectively. Other somatic mutant genes were of a relatively low frequency (<25%). The overall somatic mutation burden was relatively low. TMB status was divided into TMB-L (≤3.6/Mb) and TMB-H (>3.6/Mb) according to the median TMB threshold. And the patients with TMB-L and TMB-H were 42 cases and 40 cases, respectively. The median OS in patients with TMB-L and TMB-H was 6.5 and 4.7 years, respectively (χ 2=6.59, P=0.010). TMB status was an independent factor for OS ( HR=0.73, P=0.021). Conclusion:TMB is a biomarker for evaluating the prognosis of CRC patients after surgical resection and receiving capecitabine-based adjuvant chemotherapy .

Research Progress in Treatment of Cerebral Vascular Disease by Panax Notoginseng Saponins / 中国中医药信息杂志

Notoginseng Radix et Rhizoma has the efficacy of dissolving stasis and hemostasis and reducing swelling and easing pain. Panax notoginseng saponins (PNS) is the main active component of Notoginseng Radix et Rhizoma, and the main components include ginsenoside Rb1, Rg1, Re, Rd and notoginsenoside R1. Injections with PNS as the medicinal material basis have become main TCM injections for the treatment of cerebral vascular diseases, with confirmed clinical efficacy. This article reviewed the research progress in pharmacological effects, clinical application and adverse reactions of PNS in treatment of cerebral vascular disease, with a purpose to provide references for further research and clinical application.

The short-term efficacy of autogenous bone pate and palva graft for obliterating huge mastoid cavity in canal wall-down approach / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To observe the short-term efficacy of autogenous bone pate and Palva graft for obliterating huge remnant mastoid cavity in canal wall down approach.@*METHOD@#Retrospective analysis clinical data of twenty-one cholesteatomatous cases operated by one surgeon from 2004 to 2007. In twelve cases, simultaneous III type tympanoplasty (Sheehy, P. O. P) was performed. Other 9 cases had undergone mastoidectomy elsewhere before the admission. Six of them were still draining with huge remnant mastoid cavity, and the rest three patients had relapsed cholesteatomas with intermittent draining and huge mastoid cavity. Normal saline solution perfusion was used to measure the volume of remnant mastoid cavity. The criterion of huge remnant mastoid cavity is more than 8 ml.@*RESULT@#Of twelve primary cases with III type tympanoplasty, 11 patients maintained a small, dry, and healthy mastoid cavity after twenty-seven days. The average increase of hearing level of them was 17.5dB, and the air-bone gap is less than 20 dB. Of one patient, bone pate was infected and was discharged. A dry mastoid cavity was achieved until fifty-five days after surgery. The patient is keeping a big air-bone gap caused by displacement of ossicle chain prosthesis. Just eighteen days later, other nine cases of revision mastoidectomy achieved a small, dry, and healthy mastoid cavity, with lightly improved hearing level.@*CONCLUSION@#Obliteration of a canal wall down huge mastoid cavity by Palva graft with autologous bone pate is a reliable and effective technique that results in a small, dry, low-maintenance mastoid cavity. The short-term efficacy of simultaneous III tympanoplasty is satisfactory if patient selection is suitable.

Primary pharmacological studies on the saponins from the solid fermented roots of Radix Notoginseng produced in Yunnan province / 中华中医药杂志

Objective: To compare the pharmacodynamic action of Radix Notoginseng after fermented by different strains bioconversion.Methods:The models of cerebral ischemia and hypoxia were established,thrombosis in vivo were induced by collagen-Adr,ventricular fibrillation of mice by chloroform,and perfusion experiment of the ex vivo heart of cavia cobaya was used.Results:100,200mg/kg doses of all samples had protective effects on ischemia and hypoxia mice,1# sample can inhibit thrombosis induced by collagen-adrenaline obviously after intraperitoneal injection in dosage of 200mg?kg-1?d-1 for 5days.All groups significantly reduced the incidents of ventricullar fibrillation induced by chloroform in mice except 100mg/kg dose of 1#,furthermore,compared with control high dose group(5#),the incidents of high dose groups of 3、4# deceased significantly.All samples had the effect of dilating coronary artery,but there effects on myocardial contractile force and cardiac preload were different.Conclusion:The primary cardiovascular related pharmacological researches demonstrated the vary action changes of 1-4# samples compared with the sample 5#.This result has revealed that the chemical structure of Radix Notoginseng transformed and that is coincide with initial analysis of phytochemical analysis.